Abstract: Molecular recognition has an important role in numerous living systems. One of the most important molecular recognition methods is molecular imprinting, which allows host compounds to recognize and detect several molecules rapidly, sensitively and selectively. Compared to natural systems, molecular imprinting methods have some important features such as low cost, robustness, high recognition ability and long term durability which allows molecularly imprinted polymers to be used in various biotechnological applications, such as chromatography, drug delivery, nanotechnology, and sensor technology. Sensors are important tools because of their ability to figure out a potentially large number of analytical difficulties in various areas with different macromolecular targets. Proteins, enzymes, nucleic acids, antibodies, viruses and cells are defined as macromolecules that have wide range of functions are very important. Thus, macromolecules detection has gained great attention in concerning the improvement in most of the studies. The applications of macromolecule imprinted sensors will have a spacious exploration according to the low cost, high specificity and stability. In this review, macromolecules for molecularly imprinted sensor applications are structured according to the definition of molecular imprinting methods, developments in macromolecular imprinting methods, macromolecular imprinted sensors, and conclusions and future perspectives. This chapter follows the latter strategies and focuses on the applications of macromolecular imprinted sensors. This allows discussion on how sensor strategy is brought to solve the macromolecules imprinting.
…Read MoreIt's about, "Whole cell based microcontact imprinted capacitive biosensor for the detection of Escherichia coli"
In this study, a label-free, selective and sensitive microcontact imprinted capacitive biosensor was developed for the detection of Escherichia coli. The recognition of E. coli was successfully performed by this sensor prepared with the combination of microcontact imprinting method and capacitive biosensor technology. After prepara- tion of bacterial stamps, microcontact-E. coli imprinted gold electrodes were generated using an amino acid based recognition element, N-methacryloyl-L-histidine methylester (MAH), 2-Hydroxyethyl methacrylate (HEMA) as monomers and ethyleneglycol dimethacrylate (EGDMA) as crosslinker under UV-polymerization. Real-time E. coli detection experiments were carried out within the range of 1.0×102–1.0×107 CFU/mL. The unique combination of these two techniques provides selective detection with a detection limit of 70 CFU/mL. The designed capacitive sensor has high selectivity and was able to distinguish E. coli when present together with competing bacterial strains which are known to have similar shape. In addition, the prepared sensor has the ability to detect E. coli with a recovery of 81–97% in e.g. river water.
…Read MoreYÖK tarafından, yükseköğretim tarihinde ilk kez "KBRN (Kimyasal, Biyolojik, Radyolojik ve Nükleer) Çalıştayı" düzenlendi. YÖK Başkanı Prof. Dr. M. A. Yekta Saraç'ın başkanlığında YÖK'te gerçekleştirilen çalıştayda Sağlık Bakanlığı, Milli Savunma Bakanlığı, Kızılay, AFAD, Kamu Hastaneler Kurumu, TAEK, TÜBİTAK gibi paydaş kurumlardan temsilciler ile farklı üniversitelerden çeşitli alanlarda akademisyenler bir araya geldi. Üniversitelerimizde KBRN alanında yapılacak çalışmaların etraflıca masaya yatırıldığı çalıştayın açılış konuşmasını gerçekleştiren YÖK Başkanı Prof. Dr. M. A. Yekta Saraç ülkemizin KBRN konusunda çok önemli bir coğrafyada bulunduğunu belirterek "Yeni YÖK" olarak ülkemizin ihtiyaçları doğrultusunda yükseköğretim alanında planlamalar yapmakta olduklarını, bu bağlamda KBRN konusunda bilimsel ve akademik destek sağlanması, üniversitelerimizin ve akademisyenlerimizin aktif katılımı ile "belirleme ve savunma", ayrıca "hızlı tanı ve tedavi" konularında ülke potansiyelinin artırılmasını hedeflediklerini söyledi.
26 Nisan 2017 / Ankara
…Read MoreTÜBA, Milli Eğitim Bakanlığı (MEB) ile imzaladığı protokol çerçevesinde ve TÜBA Bilim Eğitimi Programı kapsamında; temel bilimler öğretmenlerine yönelik “V. Uygulamalı Bilim Eğitimi Kursu”, 30 Ocak-1 Şubat tarihleri arasında İstanbul Üniversitesi’nde gerçekleştirildi.
…Read More11. Ulusal Afinite Teknikleri Kongresi, 15-17 Haziran tarihleri arasında Adnan Menderes Üniversitesi katkılarıyla yapılacaktır. Başvurular için lütfen afiniteteknikleri2017.com adresini ziyaret ediniz.
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